Mechanism of Ca2+ on the hyperthermia-induced apoptosis of rat hippocampal neurons in vitro / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 185-187, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-346537
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of Ca(2+) on the apoptosis induced by hyperthermia in neonate rat hippocampal neurons to provide the applicative evidence of dantrolene for preventing brain injuries.</p><p><b>METHODS</b>Dantrolene, Ca(2+) specific blocking agent, was used in the hyperthermia-induced apoptosis of primary hippocampal neurons in vitro to observe its effect on the apoptosis, fluorescent intensity, and dynamic change of Ca(2+) by flowcytometry and laser confocal microscopy.</p><p><b>RESULTS</b>The rate of apoptosis was decreased significantly after hyperthermia treatment by dantrolene sodium. The intracellular Ca(2+) fluorescent intensity in 42 degrees C treatment group (107.35 +/- 6.0) was significantly lower than that in control group (159.12 +/- 33.8). The concentration of Ca(2+) began to decrease 20 approximately 25 s after adding dantrolene sodium, and reached the lowest level about 50 s later, and then kept lower than the basal level.</p><p><b>CONCLUSION</b>Dantrolene sodium has an important protective effect on hippocampal neurons apoptosis induced by hyperthermia and may have some applicative value of preventing heat-induced brain injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Temperature
/
Calcium Channel Blockers
/
Cells, Cultured
/
Calcium
/
Rats, Sprague-Dawley
/
Apoptosis
/
Cell Biology
/
Dantrolene
/
Hippocampus
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2005
Type:
Article
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