Investigation of the frequency of chromosomal aneuploidy using triple fluorescence in situ hybridization in 12 Chinese infertile men / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 503-506, 2004.
Article
in English
| WPRIM
| ID: wpr-346639
ABSTRACT
<p><b>BACKGROUND</b>Chromosomal aberrations are the major cause of pre- and post-implantation embryo wastage and some studies suggest that half of all human conceptions have a chromosomal abnormality. A chromosomal aberration in human sperms is also one of the causes of failure of in vitro fertilization. This study was designed to ascertain whether chromosomal aneuploidy in spermatozoa is a risk factor for male infertility.</p><p><b>METHODS</b>Twelve infertile men were divided into two groups 10 with oligoasthenoteratozoospermia (OAT, Group A) and two with a normal semen analysis (Group B). Two normal healthy sperm donors acted as controls (Group C). We used fluorescence in situ hybridization (FISH) and probes for chromosomes X, Y and 18 to determine the frequency of aneuploidy.</p><p><b>RESULTS</b>The frequencies of spermatozoa disomy for chromosomes X, Y and 18 were 0.30% and 0.30%, respectively, in Group B. The percentages were not significantly different from those of Group C (0.15% and 0.16%). The frequencies of nullisomy for chromosomes X, Y and 18 were 0.15% and 0 for Group B, and 0 and 0.15% for Group C (P > 0.05). In Group A, the incidences of disomy were 1.13% and 0.96% and the frequencies of nullisomy were 1.13% and 1.60%. In these three groups, the incidences of diploidy were 0.60%, 1.00%, and 0.30%, respectively. Both the frequencies of disomic and nullisomic spermatozoa for chromosomes X, Y, and 18 and of diploid spermatozoa were significantly higher in Group A than in Groups B and C. The estimated total aneuploidy rates in the sperm from the three groups were 42.44%, 6.05%, and 2.59%, respectively.</p><p><b>CONCLUSION</b>These results indicate that chromosomal aneuploidy in spermatozoa may be a risk factor for infertility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chromosomes, Human, Pair 18
/
Risk Factors
/
In Situ Hybridization, Fluorescence
/
Chromosomes, Human, X
/
Chromosomes, Human, Y
/
Genetics
/
Infertility, Male
/
Aneuploidy
Type of study:
Etiology study
/
Risk factors
Limits:
Adult
/
Humans
/
Male
Language:
English
Journal:
Chinese Medical Journal
Year:
2004
Type:
Article
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