Expression and purification of human amelogenin mature peptide in Escherichia coli / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 279-281, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-346748
ABSTRACT
<p><b>OBJECTIVE</b>To establish the expression and purification route for human amelogenin mature peptide in Escherichia coli and obtain the purified amelogenin (AMG) mature peptide.</p><p><b>METHODS</b>Recombined plasmid pGEX-4T-1-AMG was transformed to Escherichia coli BL21. After expression, AMG was purified with glutathione S-transferase fusion protein purification system (GSTrapFF) column.</p><p><b>RESULTS</b>Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting hybridization results showed that 45,000 GST-AMG fusing protein and 19,000 target AMG mature peptide were obtained successfully.</p><p><b>CONCLUSIONS</b>pGEX-4T-1-AMG-BL21 system is used successfully to express and purify human AMG mature peptide.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Recombinant Fusion Proteins
/
Recombinant Proteins
/
Electrophoresis, Polyacrylamide Gel
/
Escherichia coli
/
Amelogenin
/
Genetics
/
Glutathione Transferase
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2009
Type:
Article
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