Chemical constituents and their antitumor cytotoxic activity in leaves of Ilex centrochinensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 354-357, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-346818
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents in leaves of Ilex centrochinensis and their antitumor bioactivity.</p><p><b>METHOD</b>Various chromatography techniques such as column chromatography on silica gel, Sephadex LH-20 and preparative HPLC were used to isolate and purify the compounds and their structures were identified by spectral data and physicochemical properties. Their antitumor effect was tested by MTT method.</p><p><b>RESULT</b>Ten compounds were isolated and identified as 1,4-benzenediol (1), (2S)-5,4'-dihydroxy-7,3'-dimethoxyflavan(2), (2S)-5,4'-dihydroxy-7-methoxyflavan (3), kaempferol (4), quercetin (5), naringenin (6), ursolic acid (7), uvaol (8), oleanolic acid (9) and beta-sitosterols (10).</p><p><b>CONCLUSION</b>Compounds 1-5, 7, 8 were isolated from the species for the first time, among which compounds 1-3 were isolated from the Ilex genus for the first time. Compounds 2 and 3 showed strong cytotoxic activity against Huh7 cell lines with IC50 values of 8.98, 13.04 mg x L(-1), respectively. Compounds 7-9 exhibited weak cytotoxic activity against Caco-2 cell lines with IC50 values of 28.52, 38.28, 33.04 mg x L(-1), respectively.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Plant Extracts
/
Cell Survival
/
Chemistry
/
Plant Leaves
/
Caco-2 Cells
/
Inhibitory Concentration 50
/
Ilex
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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