Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3799-3802, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-346835
ABSTRACT
<p><b>OBJECTIVE</b>To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.</p><p><b>METHOD</b>HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions ODS column was adopted, with methanol-water-phosphate (47 53 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.</p><p><b>RESULT</b>S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).</p><p><b>CONCLUSION</b>The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plant Growth Regulators
/
Purines
/
Benzyl Compounds
/
Flavonoids
/
Chromatography, High Pressure Liquid
/
Scutellaria baicalensis
/
Tissue Culture Techniques
/
2,4-Dichlorophenoxyacetic Acid
/
Dose-Response Relationship, Drug
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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