PCR-SSCP molecular identification of Panax ginseng and P. quinquefolius based on ITS2 bar coding SNPs / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3748-3751, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-346845
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for identifying Panax ginseng and P. quinquefolius with PCR-SSCP, on the basis of specific SNP identification sites of their ITS2 bar codes.</p><p><b>METHOD</b>ITS2 sequences of P. ginseng and P. quinquefolius recorded in GenBank were searched to conduct a comparative analysis and screen out specific SNP identification sites of their ITS2 bar codes. Based on that, the Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method was adopted for analyzing 11 P. ginseng samples and 10 P. quinquefolius samples and verifying sequencing of their PCR products.</p><p><b>RESULT</b>The P. ginseng and P. quinquefolius samples had the same agarose mages of PCR-SSCP with the standard medicines. There were significant differences between the PCR-SSCP agarose mages of P. ginseng and P. quinquefolius, with identifical identification results between PCR-SSCP and sequencing.</p><p><b>CONCLUSION</b>Compared with the sequencing method, PCR-SSCP is so rapid and accurate that it can be used for identifying P. ginseng and P. quinquefolius medicines.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Species Specificity
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Classification
/
Polymorphism, Single-Stranded Conformational
/
DNA, Plant
/
Polymorphism, Single Nucleotide
/
DNA, Ribosomal Spacer
/
Electrophoresis, Agar Gel
/
DNA Barcoding, Taxonomic
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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