Your browser doesn't support javascript.
loading
PCR-SSCP molecular identification of Panax ginseng and P. quinquefolius based on ITS2 bar coding SNPs / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3748-3751, 2012.
Article in Chinese | WPRIM | ID: wpr-346845
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for identifying Panax ginseng and P. quinquefolius with PCR-SSCP, on the basis of specific SNP identification sites of their ITS2 bar codes.</p><p><b>METHOD</b>ITS2 sequences of P. ginseng and P. quinquefolius recorded in GenBank were searched to conduct a comparative analysis and screen out specific SNP identification sites of their ITS2 bar codes. Based on that, the Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method was adopted for analyzing 11 P. ginseng samples and 10 P. quinquefolius samples and verifying sequencing of their PCR products.</p><p><b>RESULT</b>The P. ginseng and P. quinquefolius samples had the same agarose mages of PCR-SSCP with the standard medicines. There were significant differences between the PCR-SSCP agarose mages of P. ginseng and P. quinquefolius, with identifical identification results between PCR-SSCP and sequencing.</p><p><b>CONCLUSION</b>Compared with the sequencing method, PCR-SSCP is so rapid and accurate that it can be used for identifying P. ginseng and P. quinquefolius medicines.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Species Specificity / Polymerase Chain Reaction / Reproducibility of Results / Classification / Polymorphism, Single-Stranded Conformational / DNA, Plant / Polymorphism, Single Nucleotide / DNA, Ribosomal Spacer / Electrophoresis, Agar Gel / DNA Barcoding, Taxonomic Type of study: Diagnostic study / Prognostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2012 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Species Specificity / Polymerase Chain Reaction / Reproducibility of Results / Classification / Polymorphism, Single-Stranded Conformational / DNA, Plant / Polymorphism, Single Nucleotide / DNA, Ribosomal Spacer / Electrophoresis, Agar Gel / DNA Barcoding, Taxonomic Type of study: Diagnostic study / Prognostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2012 Type: Article