HPLC determination of hydroxysafflor yellow A in Mongolian medicine Dedu Honghuaqiwei pill / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.</p><p><b>METHOD</b>The determination was performed by HPLC method on Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26 2 72) as mobile phase. The column temperature was 30 degrees C and the flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>The linear rang of hydroxysafflor yellow A was 0.068-0.408 microg and the recovery was 97.66%.</p><p><b>CONCLUSION</b>The result is accurate with good resolution, and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.</p>