Identification of corium stomachium galli by molecular taxonomy / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3192-3194, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-346920
ABSTRACT
<p><b>OBJECTIVE</b>To establish a convenient and accurate method of DNA molecular marker for the identification of corium stomachium galli.</p><p><b>METHOD</b>Cytb mtDNA sequences of Gallus gallus domestica and three other species of poultry were downloaded from Genbank. Species-specific PCR primers were designed according to the differential DNA fragments of the cytb genes. PCR tests were performed with the DNAs extracted from G. gallus domestica, three other poultry species and domestic mammal animals.</p><p><b>RESULT</b>The specific primers of G. gallus domestica could only amplify the cytb mtDNA of G. gallus domestica.</p><p><b>CONCLUSION</b>The primers are specific to G. gallus domestica mtDNA and can used to discern Corium stomachium from the false medicine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Mitochondrial
/
Chickens
/
Polymerase Chain Reaction
/
Classification
/
Genetics
/
Liver
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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