Hypoxia-A Possibility in Fibromyalgia Syndrome Pathogenesis / 대한류마티스학회지

ABSTRACT

OBJECTIVE: We studied the expression of pain-related molecules such as substance P involved in chronic pain of fibromyalgia syndrome (FMS) patients using rat cortical cells in hypoxia. METHODS: We sacrificed pregnant Sprague-Dawley rat and got embryo. We cultured the cortical cells and compared the expression of pain-related molecules in 1st, 3rd, 5th day cortical cells exposed to hypoxia (37 degrees C, 5% CO2, 98% N2) to control by immunohistochemistry. We measured the density at soma using softwear 'Scion image'. RESULTS: The expression of substance P was increased in hypoxic cortical cell group than control (control mean 49.9 vs. hypoxia 1st day 75.4 (p<0.001), 3rd day 65.6 (p<0.001), 5th day 79.9 (p<0.001)). The expression of kainate receptor was increased in hypoxic cortical cell group than control (control mean 58.4 vs. hypoxia 1st day 64.9 (p<0.001), 3rd day 63.3 (p<0.001), 5th day 62.9 (p<0.001)). The expression of N-methyl-D-aspartate receptor 2B was increased in hypoxic cortical cell group than control (control mean 59.4 vs. hypoxia 1st day 60.8 (p<0.001), 3rd day 62.6 (p<0.001), 5th day 67.1 (p<0.001)). But, the expression of calcitonin gene related peptide was decreased in hypoxic cortical cell group than control (control mean 76.8 vs. hypoxia 1st day 76.4 (p<0.001), 3rd day 71.5 (p<0.001), 5th day 61.3 (p<0.001)). CONCLUSION: Hypoxia during night could increase the expression of some pain-related molecules, which might be the cause of chronic pain in FMS patients.