Establishment of allele-specific diagnostic PCR method for identification of antlers / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3013-3016, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-346982
ABSTRACT
<p><b>OBJECTIVE</b>To establish a convenient, quick and accurate molecular method for the identification of crude drugs of antlers due to the difficult discrimination between the genuine antler and its adulterants.</p><p><b>METHOD</b>According to the alignment analysis of full length sequences of Cyth gene from closely relate species of Cervus, one pair of allele-specific diagnostic PCR primers was designed. Factors such as annealing temperature, dosage of polymerase, times of cycles and dosage of template DNA that influence the PCR results were also investigated.</p><p><b>RESULT</b>Based on the study mentioned above, about 323 bp positive band was amplified under the annealing temperature of 65 degrees C in the total volume of 25 microL PCR reaction using the genuine antler DNA as the template. Sequencing results proved that the positive band was the fragment of Cytb gene from both C. elaphus Linnaeus and C. nippon Temminck.</p><p><b>CONCLUSION</b>The established method, with higher specificity and reproducibility, could accurately differentiate genuine antler from its adulterants and would be widely used in Cervus related Chinese crude drugs' identification.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antlers
/
Species Specificity
/
Deer
/
Molecular Sequence Data
/
Chemistry
/
China
/
Polymerase Chain Reaction
/
DNA Primers
/
Alleles
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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