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Determination of chelerythrine in Chelidonium majus by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2918-2919, 2009.
Article in Chinese | WPRIM | ID: wpr-346998
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.</p><p><b>METHOD</b>Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (2575) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.</p><p><b>RESULT</b>The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.</p><p><b>CONCLUSION</b>The method is accurate, sensitive and reliable.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Chelidonium / Benzophenanthridines / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Chelidonium / Benzophenanthridines / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2009 Type: Article