A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction on endothelial cells / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 502-506, 2012.
Article
in English
| WPRIM
| ID: wpr-347171
ABSTRACT
<p><b>OBJECTIVE</b>To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction () on the endothelial cell line ECV304.</p><p><b>METHODS</b>ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction-containing serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.</p><p><b>RESULTS</b>At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed.</p><p><b>CONCLUSION</b>XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Cell Adhesion
/
Cell Line
/
Cell Movement
/
Cell Survival
/
Gene Expression Regulation
/
Neovascularization, Physiologic
/
Oligonucleotide Array Sequence Analysis
/
Endothelial Cells
Limits:
Humans
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2012
Type:
Article
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