Effects of lead exposure on acid-sensing ion channel in hippocampus of baby-rats / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 729-732, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-347229
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of chronic lead exposure on mRNA and protein expression of ASIC1a, ASIC2a, ASIC2b in hippocampus of baby-rats.</p><p><b>METHODS</b>The Wistar pregnant rats were randomly divided into 3 groups fed with distilled water or lead contained water (0.2% and 1.0% lead acetate) respectively, 5 rats in each group. The lead-exposure ranged from the 0 day of pregnancy to the offspring weaned. Then the baby-rats were fed with lead water like their mothers and killed at postnatal day 8 or 50. Atomic absorption spectrometry was used to determine lead content in the brain. RT-PCR and Western blotting were used to observe mRNA and protein expression of ASIC1a, ASIC2a and ASIC2b in their hippocampus respectively.</p><p><b>RESULTS</b>The brain lead content of test groups was higher than that of the control group (P < 0.01), and the lead content of the postnatal day 50 was higher than that in postnatal day 8 (P < 0.01). Compared with the control group, ASIC1a mRNA expression of 1.0% lead exposure in the hippocampus was uptrend (P < 0.01), ASIC1a protein expression of each test group was downtrend (P < 0.05), while for ASIC2a and ASIC2b mRNA and protein, there was no significant differences observed (P > 0.05).</p><p><b>CONCLUSION</b>ASIC1a expression in hippocampus can be changed by chronic lead exposure.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prenatal Exposure Delayed Effects
/
RNA, Messenger
/
Sodium Channels
/
Rats, Wistar
/
Toxicity
/
Acid Sensing Ion Channels
/
Genetics
/
Hippocampus
/
Lead
/
Metabolism
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2008
Type:
Article
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