Experimental study of the isolation, culture and in chondrogenic differentiation of human bone mesenchymal stem cell / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 63-66, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-347303
ABSTRACT
<p><b>OBJECTIVE</b>To study the isolation of human bone marrow mesenchymal stem cells (MSCs) and in vitro differentiation into chondrocytes as potential seed cell for condyle cartilage tissue engineering.</p><p><b>METHODS</b>Human MSCs were isolated by percoll solution from normal human bone marrow sample and cultured in flasks. Specific cell surface markers were identified by flow-cytometry. After the cells were treated with inductive medium containing insulin, transferrin, pyruvate, dexathemesone and TGF-beta for 7 - 14 days, microscopic, histological and immuno-histo-chemical studies were performed for chondrogenic phenotype identification.</p><p><b>RESULTS</b>Primary cultures of human MSCs express CD29 and CD44 positively and meanly, but CD34, CD45 and HLA-DR negatively. After 14 days of induction, the cells were positively stained by safranin O. Immunohistochemical analysis proved strong type II collagen expression.</p><p><b>CONCLUSIONS</b>Percoll helps to generate a better isolation of MSCs from human bone marrow aspirates with a purity more above 95%. The isolated MSCs can be expanded and induced in vitro to differentiate into chondrocytes by inductive medium.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyruvates
/
Stem Cells
/
Transferrin
/
Bone Marrow Cells
/
Dexamethasone
/
Immunohistochemistry
/
Cartilage, Articular
/
Cell Differentiation
/
Cells, Cultured
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2003
Type:
Article
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