Relation between activation of NF-kappa B and chemotherapy induced apoptosis of leukemic cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 216-219, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-347458
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the relation between activation of NF-kappa B and chemotherapy induced apoptosis of leukemic cells and the effect of vincristine (VCR) on them.</p><p><b>METHODS</b>Electrophoretic mobility shift assay (EMSA) was used to detect the activation of NF-kappa B and tunel DNA electrophoresis was adopted to observe the apoptosis induced by cytosine arabinoside (Ara-C) and etopside (Vp-16) in P388 leukemic cells.</p><p><b>RESULTS</b>The activation of NF-kappa B induced by Ara-C and Vp-16 was obviously correlated to apoptosis in P388 cells. VCR (0.1 micromol/L) could suppress activation of NF-kappa B by 52% and 63% and significantly increase the apoptosis by 89% and 123% as induced by Ara-C (100 micromol/L) and Vp-16 (100 micromol/L). The activity of NF-kappa B could be found in P388 cells before being exposed to chemotherapeutic agent.</p><p><b>CONCLUSION</b>Chemotherapeutic agents can induce apoptosis and activation of NF-kappa B of P388 cells. The mechanism of VCR potentiating chemotherapeutics induction of leukemia cell apoptosis may be related to its suppression of the NF-kappa B activity in the P388 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Vincristine
/
Leukemia P388
/
NF-kappa B
/
Apoptosis
/
In Situ Nick-End Labeling
/
Drug Therapy
/
Metabolism
/
Antineoplastic Agents, Phytogenic
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2003
Type:
Article
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