Effects of Ucf-101 on expression of Omi/HtrA2 in kidneys of postasphyxial neonatal rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 658-661, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-347518
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of serine protease Omi/HtrA2 in kidneys of postasphyxial neonatal rats, and to study the effects of Ucf-101 on apoptosis and the expression of Omi/HtrA2 in these rats.</p><p><b>METHODS</b>Seventy-two neonatal Wistar rats of 7-10 days old were randomly divided into 3 groups control, postasphyxial model, Ucf-101-treated postasphyxialThe postasphyxial model was established by normobaric asphyxiaExpression of Omi/HtrA2 was determined with streptavidin-peroxidase immunohistochemistry 2, 24 and 48 hrs after asphyxia. Terminal deoxynuleotidyl-mediated nick end labeling (TUNEL) was used to ascertain the apoptosis of renal cells.</p><p><b>RESULTS</b>Compared with the control group, OmiHtrA2 expression in renal cells began to increase 2 hrs after asphyxia and peaked at 24 hrs. The expression of Omi/HtrA2 in the Ucf-101-treated postasphyxial group was significantly lower than that in the postasphyxial model group (P<0.01). TUNEL-positive cells began to increase 2 hrs after asphyxia and peaked at 24 hrs in the postasphyxial model group when compared with the control group. The number of TUNEL-positive cells in the Ucf-101-treated postasphyxial group was significantly lower than that in the postasphyxial model group at all time points (P<0.01).</p><p><b>CONCLUSIONS</b>The expression of Omi/HtrA2 in kidneys is increased in postasphyxial neonatal rats. The increased Omi/HtrA2 expression may play an important role in the development of postasphyxial renal injury. Treatment with Ucf-101 can reduce the expression of Omi/HtrA2 in kidneys of postasphyxial neonatal rats and thus reduce renal tububar epithelial cell apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Pyrimidinones
/
Asphyxia Neonatorum
/
Thiones
/
Immunohistochemistry
/
Serine Endopeptidases
/
Chemistry
/
Rats, Wistar
/
Apoptosis
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2010
Type:
Article
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