Construction of tissue-engineering skin with carrier of active composite dermal matrix / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 272-274, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-347603
ABSTRACT
<p><b>OBJECTIVE</b>To construct of tissue engineering skin including active composite dermal matrix.</p><p><b>METHODS</b>The human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.</p><p><b>RESULTS</b>The structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.</p><p><b>CONCLUSION</b>Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Swine
/
Transplantation
/
Skin, Artificial
/
Cell Culture Techniques
/
Dermis
/
Cell Biology
/
Collagen Type I
/
Tissue Engineering
/
Epidermis
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2008
Type:
Article
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