Study on Acinetobacter baumannii plasmid with 3 types of beta-lactamase genes in a burn ward / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 97-98, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-347633
ABSTRACT
<p><b>OBJECTIVE</b>To study the transferrable character of Acinetobacter baumannii (AB) plasmids with 3 types of beta-lactamase gene.</p><p><b>METHODS</b>The plasmid of multi-drug resistant AB (donor) isolated from burn wound were transferred to E. coil ATCC25922 (receptor) through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR.</p><p><b>RESULTS</b>The drug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam, and three types of beta-lactamase gene were transferred to the receptor, and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was > 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation.</p><p><b>CONCLUSION</b>bla(TEM-1), bla(PER-1) and bla(OXA-23) genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages, and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Beta-Lactamases
/
Burns
/
Acinetobacter Infections
/
Microbial Sensitivity Tests
/
Drug Resistance, Multiple, Bacterial
/
Acinetobacter baumannii
/
Genes, Bacterial
/
Genetics
/
Microbiology
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2008
Type:
Article
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