Expression of matrix metalloproteinase-2, -9 and their inhibitor-1 in hypertrophic scars / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 444-446, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-347656
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the gene expression of matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in proliferative and mature hypertrophic scars.</p><p><b>METHODS</b>Total RNA from 8 normal skin samples and from 16 human hypertrophic scar samples of different maturing stage was respectively extracted, and then mRNA was isolated. The gene expressions of MMP-2, MMP-9 and TIMP-1 in these samples were examined with reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The gray scale ratio of MMP-2, MMP-9 and TIMP-1 transcription in normal skin were (3.8 +/- 0.7)%, (5.8 +/-4.4)%, (30.3 +/- 3.0)%, respectively, which were obviously higher than those in proliferative hypertrophic scar [(14 +/- 5)%, (18 +/- 5)%, (38 +/- 4)%, P < 0.05]. The expression of MMP-2 and MMP-9 genes in mature hypotrophic scar returned to normal level, but that of TIMP-1 remained high when compared with that of normal level (P < 0. 05).</p><p><b>CONCLUSION</b>The increase in MMP-2, MMP-9 and TIMP-1 gene expression might be involved in the formation of hypertrophic scars, while the lowering of MMP-2 and MMP-9 gene expression might be associated with the maturation of hypertrophic scars.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Skin
/
RNA, Messenger
/
Gene Expression
/
Cicatrix, Hypertrophic
/
Tissue Inhibitor of Metalloproteinase-1
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 9
/
Genetics
/
Metabolism
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2007
Type:
Article
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