Detection of chromosomal aberration in paraffin-embedded synovial sarcoma tissues by fluorescence in-situ hybridization / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 577-581, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-347725
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the application of fluorescence in-situ hybridization (FISH) in detection of gene translocation in paraffin-embedded tissue samples of synovial sarcoma.</p><p><b>METHODS</b>Interphase FISH was carried out in paraffin-embedded tissue of 42 cases of synovial sarcoma and 9 cases of non-synovial sarcoma, using a LSI SYT (18q11.2) dual color break-apart probe. In all of the cases studied, the gene fusion product SYT-SSX was also analyzed by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Positive signals were detected in 37 cases (88.1%) of synovial sarcoma by FISH, as compared with 35 cases (83.8%) by RT-PCR and 39 cases (92.9%) by both techniques. Of the 39 positive cases, 33 cases (78.5%) revealed SYT gene translocation.</p><p><b>CONCLUSIONS</b>FISH may serve as an adjunctive diagnostic tool in problematic cases of synovial sarcoma and can be applied in paraffin-embedded tissue samples. As compared with RT-PCR, FISH is also sensitive and reliable. The methodology is less labor intensive and time consuming. FISH has great potential in molecular diagnosis of soft tissue tumors.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Soft Tissue Neoplasms
/
Biomarkers, Tumor
/
Oncogene Proteins, Fusion
/
Chromosome Aberrations
/
Paraffin Embedding
/
In Situ Hybridization, Fluorescence
/
Sarcoma, Synovial
/
Reverse Transcriptase Polymerase Chain Reaction
/
Lower Extremity
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2007
Type:
Article
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