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The mechanisms of p21WAF1/Cip-1 expression in MOLT-4 cell line induced by TSA / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 174-181, 2005.
Article in Chinese | WPRIM | ID: wpr-347801
ABSTRACT
To investigate the function and molecular mechanism of p21(WAF1/Cip-1) expression in MOLT-4 cells induced by HDAC inhibitor TSA, the expression pattern of p21(WAF1/Cip-1) and the distribution of cell cycle in TSA treated cells were analyzed. The results showed that TSA could effectively induce G(2)/M arrest and apoptosis of MOLT-4 cells. Kinetic experiments demonstrated that p21(WAF1/Cip-1) were upregulated quickly before cell arrested in G(2)/M and began decreasing at the early stage of apoptosis. Meanwhile, the proteasome inhibitor MG-132 could inhibit the decrease of p21(WAF1/Cip-1) at the early stage of apoptosis, which showed that proteasome pathway involved in p21(WAF1/Cip-1) degradation during the TSA induced G(2)/M arrest and apoptosis responses. This study also identified that the protein level of p21(WAF1/Cip-1) was highly associated with the cell cycle change induced by TSA. Compared to cells treated by TSA only, exposure MOLT-4 cells to TSA meanwhile treatment with MG-132 increased the protein level of p21(WAF1/Cip-1) and increased the numbers of cell in G(2)/M-phase, whereas the cell apoptosis were delayed. It is concluded that p21(WAF1/Cip-1) plays a significant role in G(2)/M arrest and apoptosis signaling induced by TSA in MOLT-4 cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Leukemia, Myeloid / Cell Cycle / Blotting, Western / Apoptosis / Cell Line, Tumor / Enzyme Inhibitors / Cyclin-Dependent Kinase Inhibitor p21 / Histone Deacetylase Inhibitors Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Leukemia, Myeloid / Cell Cycle / Blotting, Western / Apoptosis / Cell Line, Tumor / Enzyme Inhibitors / Cyclin-Dependent Kinase Inhibitor p21 / Histone Deacetylase Inhibitors Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2005 Type: Article