Peripheral blood T cell immuno-tolerance in PBSCT donors induced by rhG-CSF in vivo / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 16-19, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-347835
ABSTRACT
The study was aimed to investigate the mechanism of T cell tolerance in human peripheral blood induced by rhG-CSF in vivo. Dendritic cell (DC) subsets, CD8(+)CD28(-) T suppressor cells and the expression of CD28 on T cells of peripheral blood before and after mobilization were analyzed by multicolor flow cytometry. The results showed that after mobilization by rhG-CSF in vivo, the relative counts of CD3(+)CD28(+) cells increased significantly (P < 0.01), and so did the CD8(+)CD28(+) cells (P < 0.01). The mean fluorescence intensity of CD28 expression on CD3(+) cells decreased greatly (P < 0.05), but there were no significant changes of the relative fluorescence intensity of CD28 overall expression on T cells (P > 0.05). The percentages of DC2 before mobilization were significantly lower as compared with normal bone marrow (P < 0.01). After using rhG-CSF, the DC2 count was significantly higher in the apheresis graft than in peripheral blood and bone marrow before mobilization (P < 0.01), while the DC1DC2 ratios were lower (P < 0.01) and there was no significant difference of DC1 before and after mobilization (P > 0.05). The percentages of CD8(+)CD28(-) T suppressor cells increased significantly also after mobilization (P < 0.05). It is concluded that the higher numbers of DC2 and CD8(+)CD28(-) T suppressor cells in peripheral blood grafts may contribute to the ability of tolerance in peripheral blood T cells induced by rhG-CSF in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Tissue Donors
/
Dendritic Cells
/
Recombinant Proteins
/
T-Lymphocytes
/
Granulocyte Colony-Stimulating Factor
/
Hematopoietic Stem Cell Mobilization
/
Peripheral Blood Stem Cell Transplantation
/
Allergy and Immunology
/
Immune Tolerance
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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