Establishment and evaluation on the method for platelet intracellular trehalose determination / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 837-840, 2004.
Article
in Zh
| WPRIM
| ID: wpr-347850
Responsible library:
WPRO
ABSTRACT
This study was aimed to establish an applicable, convenient and rapid method for platelet intracellular trehalose determination, so that the technique of trehalose loading could be optimized and used in research on freeze-drying platelets. Protein from loaded-trehalose platelets was deposited by using trichloroacetic acid, trechalose concentration in platelets was determinated by sulfuric-anthrone reaction and was analyzed by high performance liquid chromatography (HPLC). The results showed that when platelets were loaded with 1.7% of trehalose in loading solution, intracellular concentration determinated by sulfuric-anthrone reaction was 0.22% and derterminated by HPLC was 0.2%. The recovery rate of trehalose determinated by sulfuric-anthrone reaction was 100.7% and stability and repeatability of results were good. In conclusion, the method is convenient, rapid, accurate and highly sensitive for determination of platelet intracellar trehalose.
Full text:
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Index:
WPRIM
Main subject:
Sulfuric Acids
/
Trehalose
/
Blood Platelets
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Methods
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article