Inhibition effect of bone marrow mesenchymal stem cells on T-lymphocyte proliferation through up-regulation of CD8+CD28- T cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 666-669, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-347889
ABSTRACT
The aim of this study was to explore effect of CD8+CD28- T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+ T cells were sorted by magnetic beads; the T-lymphocyte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28- T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhibitory effect depended on the amount of MSC; the data of FACS indicated that in the CD8+ T cells co-cultured with MSC, CD8+CD28- T cells were up-regulated significantly, compared with the non-treated CD8+ T cells. In conclusion, MSC perform their immunosuppressive function by up-regulation of CD8+CD28- T cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Bone Marrow Cells
/
Lymphocyte Activation
/
Up-Regulation
/
T-Lymphocyte Subsets
/
CD8 Antigens
/
CD28 Antigens
/
Allergy and Immunology
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS