Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2.</p><p><b>METHODS</b>Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing.</p><p><b>RESULTS</b>The cloned DNA was confirmed to be hBMP-2 gene.</p><p><b>CONCLUSION</b>In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis.</p>