Effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in polarized osteoclasts / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts.</p><p><b>METHODS</b>Rat polarized osteoclasts suffered 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real-Time fluorescent quantitation PCR.</p><p><b>RESULTS</b>mRNA expression of ATP6V1a1 in 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 groups was (1.14 +/- 0.06) x 10(6), (1.62 +/- 0.09) x 10(6), (2.28 +/- 0.13) x 10(6), (3.24 +/- 0.18) x 10(6), (9.16 +/- 0.53) x 10(6) copy numbers, respectively (P < 0.05).</p><p><b>CONCLUSION</b>In the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength.</p>