Targeting transgene effect of adTERT-TRAIL on salivary adenoid cystic carcinoma cell line SACC-83 / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the targeting expression of TRAIL gene driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell with telomerase activity.</p><p><b>METHODS</b>Adenovirus vector AdTERT-TRAIL was constructed by homologus recombination. After transfecting AdTERT-TRAIL into SACC-83 cell and HEL cell, its effect on these cells in vitro was investigated using RT-PCR technique, MTT method and flow cytometry.</p><p><b>RESULTS</b>After transfection of AdTERT-TRAIL, expression of extrinsic TRAIL gene driven was detected in SACC-83, the proliferation of SACC-83 cell showed significant inhibitory effect (the relative cell viability was 49.70%) and its apoptotic rate was promoted (30.49%), whereas no TRAIL gene was detected in HEL cell, also no inhibitory effect was observed in HEL cell and its apoptotic rate showed little change.</p><p><b>CONCLUSION</b>Adenovirus vector AdTERT-TRAIL was successfully constructed, which can be used to induce expression of TRAIL gene in SACC-83 cell with targeting effect.</p>