Nasal immunization with co-expression plasmid harboring genes encoding porphyromonas gingivalis FimA and human interleukin-15 in mice / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 177-179, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-348070
ABSTRACT
<p><b>OBJECTIVE</b>To observe the antibody responses induced by recombinant plasmid plRES-fimAIL15 via nasal immunization to BABL/c mice and the regulation of IL-15 to sIgA.</p><p><b>METHODS</b>BABL/c mice were immunized with recombinant plasmids pIRES-fimAIL15 and pIRES-fimA via nasal or intramuscular route. Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA.</p><p><b>RESULTS</b>Nasal immunization with plasmids pIRESfimAIL15 or pIRES-fimA elicited significant higher level of salivary FimA-specific sIgA responses compared with intramuscular immunization. There was no significant difference of the serum IgG responses between nasal immunization mice and intramuscular immunization mice. Nasal immunization with plasmid pIRES -fimAIL15 elicited significant higher level of salivary sIgA response than with pIRES-fimA (P<0.05).</p><p><b>CONCLUSION</b>Nasal dropping may be an effective mucosal immunization route of anti-Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region. IL-15 has a positive regulation effect to sIgA response.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Immunization
/
Porphyromonas gingivalis
/
Interleukin-15
/
Mice, Inbred BALB C
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2007
Type:
Article
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