Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 738-741, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-348196
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer.</p><p><b>METHODS</b>A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test.</p><p><b>RESULTS</b>When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred.</p><p><b>CONCLUSION</b>Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Pathology
/
RNA, Messenger
/
Transfection
/
Down-Regulation
/
Gene Expression Regulation, Neoplastic
/
Cell Cycle
/
Apoptosis
/
Annexin A1
/
RNA, Small Interfering
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article
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