Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 579-581, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-348725
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hepatitis C virus (HCV) non-structure protein NS5A on the activity of calcium-regulating protein alpha subunit of nascent polypeptide-associated complex (NACA) promoter.</p><p><b>METHODS</b>HepG2 cell plasmid pCAT3-NACA, containing NACA promoter, was transfected alone or cotransfected with pcDNA3.1(-)-NS5A, and chloramphenicol acetyl transferase (CAT) enzyme activity was assayed by enzyme-linked immunoassay (ELISA).</p><p><b>RESULTS</b>The CAT activity in the pcDNA3.1(-)-NS5A cotransfection group was 20.7% of the CAT activity in the pCAT3-NACA group.</p><p><b>CONCLUSION</b>HCV non-structural protein NS5A has a down-regulating effect on the promoter of NACA gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Molecular Sequence Data
/
Base Sequence
/
Transfection
/
Down-Regulation
/
Transcriptional Activation
/
Promoter Regions, Genetic
/
Viral Nonstructural Proteins
/
Molecular Chaperones
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2005
Type:
Article
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