Immune response enhanced by genes encoding IFN-alpha 8 and T alpha 1 co-inoculated with HBV DNA vaccine / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 497-500, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-348754
ABSTRACT
<p><b>OBJECTIVES</b>To evaluate if the humoral immune response of hepatitis B DNA vaccine pVAX1-S2S could be enhanced by Talpha1 and/or IFNa expression plasmid co-inoculated.</p><p><b>METHODS</b>The following mammalian expression recombinant plasmids were constructed the plasmid pVAX1-S2S expressing hepatitis B surface antigen S2S, the plasmid pVAX1-T/I co-expressing thymosin a and IFNalpha, the plasmid pVAX1-I/S2S co-expressing IFNalpha and S2S. These plasmids were inoculated intramuscularly into several BALB/c mice groups in different combinations. In the co-immunization group 1 (pVAX1-I/S2S), each mouse was inoculated with 100 microg of pVAX1-I/S2S; in the co-immunization group 2 (pVAX1-S2S) each mouse was co-inoculated with pVAX1-S2S and 50 microg of pVAX1-TI; in the control group each mouse was inoculated with 100 microg of pVAX1-S2S. All the immunizations were boosted at 2 and 4 week intervals; then the serum samples were collected to detect the anti-HBs and anti-preS2 strengths.</p><p><b>RESULTS</b>3, 5 and 8 weeks after the first inoculation, the positive rates of anti-HBs were 12.5%, 12.5%, 62.5% respectively in the co-immunization group 1 and 25%, 50%, 50% in the co-immunization group 2, while those in the control group were 0, 25%, 37.5%. The titers of anti-preS2 in co-immunization group 2 was 5 times higher than those in the other two groups.</p><p><b>CONCLUSION</b>The data shows that Talpha1 and/or IFNalpha expression plasmid co-inoculated with pVAX1-S2S might act as an adjuvant to enhance the humoral immune response induced by pVAX1-S2S.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Thymosin
/
Recombinant Fusion Proteins
/
Adjuvants, Immunologic
/
Interferon-alpha
/
Hepatitis B Vaccines
/
Vaccines, DNA
/
Therapeutic Uses
/
Allergy and Immunology
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2005
Type:
Article
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