Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with different metastasis potentials / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 436-439, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-348781
ABSTRACT
<p><b>OBJECTIVES</b>To compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence.</p><p><b>METHODS</b>Using two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials.</p><p><b>RESULTS</b>10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I.</p><p><b>CONCLUSION</b>The changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Electrophoresis, Gel, Two-Dimensional
/
Carcinoma, Hepatocellular
/
Phosphotyrosine
/
Cell Line, Tumor
/
Liver Neoplasms
/
Metabolism
/
Neoplasm Metastasis
/
Neoplasm Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2005
Type:
Article
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