Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 428-431, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-348783
ABSTRACT
<p><b>OBJECTIVES</b>To study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Fifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using real-time quantitative PCR (RQ-PCR).</p><p><b>RESULTS</b>The expression level of DLC-1 mRNA in HCC specimens with high invasiveness was significantly lower than that with low invasiveness (P < 0.05). The expression levels of DLC-1 mRNA were significantly different between non-metastatic (Hep3B and HepG2) and metastatic (MHCC97-H, MHCC97-L and HCCLM3) cell lines (P < 0.05). From MHCC97-L to HCCLM3, with an increase of invasiveness and metastatic potentials, the expression level of DLC-1 decreased correspondingly, and its expression level in HCCLM3 was significantly lower than that in MHCC97-L (P < 0.01).</p><p><b>CONCLUSION</b>The expression of DLC-1 mRNA may play an important role in inhibiting the invasiveness and metastasis of HCC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Mutagenesis, Site-Directed
/
Carcinoma, Hepatocellular
/
GTPase-Activating Proteins
/
Tumor Suppressor Proteins
/
Cell Line, Tumor
/
Genetics
/
Liver Neoplasms
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2005
Type:
Article
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