The 102 kb pigmentation (pgm) locus of Yersinia pestis isolated from Microtus brandti / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 291-295, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-348846
ABSTRACT
<p><b>OBJECTIVE</b>To find out the differences between 102 kb pgm locus of Yersinia pestis isolated from Microtus brandti with of other types, and the characters of Yersinia pestis isolated from Microtus brandti caused by their makeup of the 102 kb pgm locus.</p><p><b>METHODS</b>102 kb pgm locus of Yersinia pestis isolated from Microtus brandti and Yersinia pestis isolated from Marmota himalayana were amplified by polymerase chain reation (PCR) with 25 pair of nested primers. The PCR products of one pair of primer were obviously different, and then cloned and sequenced. Sequences were searched against current protein and nucleotide databases, using BLAST.</p><p><b>RESULTS</b>The 102 kb pgm locus of Yersinia pestis isolated from Microtus brandti was devoid one IS100. In addition, it had more copies than other types in the similar variable-number tandem repeat sequences.</p><p><b>CONCLUSION</b>The 102 kb pgm locus of Yersinia pestis was different from that of other types. It had only one IS100 flanked it, which corresponded to the character that its pgm(+) phenotype was stable. Further study was needed to confirm the relationship between the diminution virulence of Yersinia pestis isolated from Microtus brandti and the loss of IS100 and other changes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pigments, Biological
/
Plague
/
Bacterial Proteins
/
Virulence
/
Yersinia pestis
/
DNA, Bacterial
/
Repetitive Sequences, Nucleic Acid
/
China
/
Polymerase Chain Reaction
/
Epidemiology
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2003
Type:
Article
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