Establishment and drug susceptibility test of isoniazid resistant Mycobacterium smegmatis / 药学学报
Acta Pharmaceutica Sinica
;
(12): 377-382, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-348945
ABSTRACT
With the emergence of drug resistant tuberculosis, it is very urgent to find novel anti-tuberculosis drugs, especially novel anti-drug-resistant tuberculosis drugs. Because of the slow growth and the need to work in a biosafty environment of Mycobacterium tuberculosis, the development of evaluation of drug effect is severely impeded. In order to solve these issues, non-pathogenic fast-growing Mycobacterium smegmatis is introduced as test organism. The inhA is one of a target of isoniazid (INH) overexpression or mutation of this gene in Mycobacterium tuberculosis conferring resistant to INH. A recombinant plasmid bearing inhA was constructed and electroporated into Mycobacterium smegmatis, using shuttle expression vector pMV261. Transformants were induced to express a protein of inhA, identified by SDS-PAGE. Results show that Mycobacterium smegmatis containing inhA plasmids exhibited 100-fold or greater increased resistance to INH, but it conferred no increased resistance to others first-line anti-tuberculosis drugs. Resazurin microtiter assay plate testing of Mycobacterium smegmatis susceptibility to drugs is a rapid, simple, and inexpensive method and could decrease color background of drugs by detecting fluorescence. It will be benefit for high-throughout screening of drugs of anti-isoniazid-resistant Mycobacteria.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidoreductases
/
Pharmacology
/
Plasmids
/
Rifampin
/
Bacterial Proteins
/
Streptomycin
/
Microbial Sensitivity Tests
/
Electroporation
/
Mycobacterium smegmatis
/
Drug Resistance, Bacterial
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Type:
Article
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