Determination of protein binding rate of oleanolic acid in human plasma and serum albumin / 药学学报
Acta Pharmaceutica Sinica
;
(12): 243-246, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-348970
ABSTRACT
A LC-MS method was established for the determination of the protein binding rates of oleanolic acid in human plasma and serum albumin. The equilibrium dialysis combined with LC-MS to determine the total concentration in plasma and free drug concentration of oleanolic acid was carried out. The human plasma protein binding rates of oleanolic acid at three concentrations were 79.6%, 81.9% and 63.3%, respectively. The human serum albumin protein binding rates of oleanolic acid at three concentrations were 53.5%, 56.6% and 47.7%, respectively. The method is shown to be simple, accurate, sensitive and specific for the determination of biological samples. The protein binding rates in human plasma and serum albumin were of high strength.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oleanolic Acid
/
Protein Binding
/
Mass Spectrometry
/
Blood
/
Serum Albumin
/
Chromatography, Liquid
/
Sensitivity and Specificity
/
Dialysis
/
Metabolism
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Type:
Article
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