Study of a novel compound 2460A with activities produced by fungus / 药学学报
Acta Pharmaceutica Sinica
; (12): 165-169, 2011.
Article
in Zh
| WPRIM
| ID: wpr-348981
Responsible library:
WPRO
ABSTRACT
With IL-6R as target, a new compound 2460A was identified from fungus using HTS screening model. The taxonomics of the produced strain was confirmed to be Trichoderma hazianum rifai after sequencing analysis of rDNA-ITS (internal transcribed spacer). Results showed that this compound has a binding activity on IL-6R competed with IL-6, thus it is a new ligand of IL-6R originating from microbe. With MTT assay, the anti-tumor activities of 2460A were demonstrated on CM126 and HT-29 cell lines separately, the IC50 are 2.17 x 10(-5) mol x L(-1) and 1.8 x 10(-5) mol x L(-1) respectively. The compound affected lightly the HT-29 cell cycle at S phase. Studies for the anti-tumor activity of 2460A in vivo are in progress in our lab.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Trichoderma
/
Binding, Competitive
/
Cell Cycle
/
Chemistry
/
Interleukin-6
/
HT29 Cells
/
Bone Marrow Neoplasms
/
Receptors, Interleukin-6
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Type:
Article