Application of multicolor primed in situ labeling in simultaneous identification of chromosomes 18, X and Y in uncultured amniocytes / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 439-442, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-349058
ABSTRACT
<p><b>OBJECTIVE</b>To establish a multicolor primed in situ labeling (PRINS) protocol for chromosome detection in uncultured amniocytes.</p><p><b>METHODS</b>Chromosomes 18, X and Y in uncultured amniocytes were simultaneously detected by using the non-ddNTP-blocking multicolor PRINS procedure.</p><p><b>RESULTS</b>Within 7 h, the 3 chromosomes were simultaneously marked in the same uncultured amniocyte. The chromosome signals were successfully detected in 69 uncultured samples of amniotic fluid. The results were consistent with that obtained by chromosomes in cultured amniocytes.</p><p><b>CONCLUSION</b>This multicolor protocol was high throughput, fast, simple, sensitive and reliable in diagnosing chromosome abnormalities in uncultured amniocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prenatal Diagnosis
/
Chromosomes, Human, Pair 18
/
Cells, Cultured
/
Chemistry
/
In Situ Hybridization, Fluorescence
/
Primed In Situ Labeling
/
Cell Biology
/
Chromosomes, Human, X
/
Chromosomes, Human, Y
/
Genetics
Type of study:
Diagnostic study
/
Practice guideline
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2009
Type:
Article
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