Two-dimensional gel electrophoresis of subcellular fractions of hepatoma cells / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVES</b>To seek a better profiling of proteins of hepatoma cells.</p><p><b>METHODS</b>The homogenate of hepatoma cells QGY-7703 was fractionated into four parts by differential centrifugation the nuclei, the pellet by 20,000 x g, the pellet by 100,000 x g and the cytosolic supernatant. The four fractions were submitted to two-dimensional gel electrophoresis and their electrophoretic patterns were analyzed.</p><p><b>RESULTS</b>In comparison with the protein pattern of hepatoma cells not fractionated, the patterns of the four fractions display many more protein spots, and a large number of proteins present in the nuclei and cytosolic supernatant were not shown in the not-fractionated samples.</p><p><b>CONCLUSION</b>Preparation of subcellular fractions before electrophoretic procedures proves to be very useful; not only can it improve the results of two-dimensional gel electrophoresis, but also can lead to research into the subcellular level.</p>