Assessment of the estrogenicity of endosulfan and other chemicals with the in vitro proliferation of human breast cancer cell / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 281-283, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-349375
ABSTRACT
OBJECTIVE:
To develop a rapid screening method for xenoestrogens and to screen the estrogenicity of some environmental chemicals.METHODS:
The E-SCREEN test was developed based on proliferation of human breast cancer cells (MCF-7), and the estrogenicity of diethylstilbestrol, 4-hydrotamoxifen and endosulfan was assessed.RESULTS:
The E-SCREEN detected estradiol at very low concentration as 1x10(-13) mol/L. It was found that diethylstilbestrol was a full agonist of estrogen receptor, endosulfan was a partial agonist, while 4 hydrotamoxifen lacked estrogenic effects at this assay.CONCLUSION:
The E-SCREEN test is sensitive, rapid, easy to perform and, therefore, suitable for large scale screening for estrogenicity of environmental chemicals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article
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