Augmentation of rat liver microsome glutathione S-transferase preparation / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 429-432, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-349427
ABSTRACT
OBJECTIVE:
To investigate the augmentation of microsomal glutathione S-transferase (mGST) preparation using simple organic compounds.METHODS:
Rat liver microsomes were isolated using both the polyethyleneGlyco 6000 (PEG6000) and Ca(2+) precipitation methods. Next the mGST activity was measured after incubation with the alkylating agent N-ethylmaleimind (NEM).RESULTS:
The baseline mGST activity of rat microsomes was 0.15 after PEG6000 exposure and 0.082 after Ca(2+) precipitation. After NEM treatment mGST activity increased to 1.797 (2.35X) (P<0.01) and 2.375 (4.127X) (P<0.01) respectively followed by purified washing. mGST activation was stimulated maximally by 5-10 mmol/L NEM and occurred rapidly with 1-2 min of co- incubation.CONCLUSION:
For both the PEG6000 and Ca(2+) precipitation methods the mGST activity of rat microsomes can be significantly enhanced after exposure to NEM. This enhancement is more prominent with the Ca(2+) precipitation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article
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