Signal transduction pathways induced by nitric oxide in rat hepatocytes / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 424-428, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-349428
ABSTRACT
OBJECTIVE:
To study signal transduction pathways in cultured rat hepatocytes in the high nitric oxide (NO) environment of hepatitis.METHODS:
NO levels were assessed by measurement of its stable oxidative products nitrite (NO2(-)) and nitrate (NO3(-)) using the Griess method with or without thiols (GSH or L-Cys). Rat hepatocytes were incubated with Sodium Nitroprusside (SNP) to produce a high NO environment and the intracellular cGMP and s-nitrosoglutathione (GSNO) in the culture media were measured using radioimmunoassay or with the MTT assay absorbed at 334nm respectively.RESULTS:
After incubation of 1.543 mmol/L SNP for 30 minutes 0.63+/-0.06 mmol/L and at 25 minutes 0.98+/-0.11 mmol/L of NO was released in containing 25 mmol/L GSH and L-Cys condition. The levels of both cGMP and GSNO were significantly increased (compared with control P<0.05) in a dose related manner.CONCLUSION:
Signal transduction of cultured rat hepatocytes in a high NO environment could be a cGMP-dependent as well as a non-cGMP-dependent pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2002
Type:
Article
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