A comparative study of computer assistant sperm analysis with rontine sperm analysis / 浙江大学学报·医学版

ABSTRACT

OBJECTIVE: To compare computer assisted sperm analysis (CASA) with routine sperm analysis (RSA) in evaluation of male fertility. METHODS: The results of CASA were compared with the RSA of 27 males normal fertility and 317 males with infertility. RESULTS: Using the CASA, deformity rate was lower than RSA, it was 25.10+/-8.57 and 31.48+/-12.81 in the fertile and infertile groups respectively, whereas, RSA was 35.56+/-9.58 and 54.56+/-13.47. Fertile group motility had a significant difference, it was 61.35+/-7.62 and 83.96+/-6.67. Sperm vitality of grades A was 9.10+/-5.50 and 30.33+/-8.85. The vitality of grades B was 31.16+/- 12.35 and 49.93+/-8.56, the vitality of grades C awas 21.06 +/-14.19,and 3.70+/-2.12, the vitality of grades D was 38.66+/-7.62 and 16.04+/-6.67. When two techniques were adopted, the mobility had no difference in infertile group (42.53+/-14.95 and 43.09+/-13.96), the sperm vitality was decreased in grades B (CASA 22.67+/-14.96,RSA 33.32+/-13.47), increased in grades C(CASA 17.08+/-13.47,RSA 7.68+/-5.82), they had significant differences (P<0.01). The grade B sperms in the non-forward moving group, CASA was 2.77+/-6.01, RSA 0. In addition, VSL, VCL, VAP, ALH, sperm trace and static diagram were adopted. CONCLUSION: Using the CASA,the distinguished ability to sperm shape and vigor was lower than RSA, but the quantize marker (such as straight line velocity, curved velocity, average path velocity and ALH) and moving, static images could reflect on sperms quality more objectively.