IFN-γ stimulation enhances immunosuppressive capability of human umbilical cord mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 605-611, 2014.
Article
in Zh
| WPRIM
| ID: wpr-349662
Responsible library:
WPRO
ABSTRACT
This study was objective to explore the effect of IFN-γ on immunosuppressive capability of mesenchymal stem cells (MSC) derived from umbilical cord. The immunomodulating capability of MSC was changed by stimulating cell surface receptors like Toll-like receptors (TLR). The inhibition of T-lymphocyte proliferation by MSC was tested via cell co-cultures. Further RT-PCR and ELISA were performed to examine the expression changes in gene and protein level. The results showed that the IFN-γ could promote the immunosuppressive effect of umbilical cord derived MSC. IFN-γ-stimulated MSC could suppress the proliferation of T cells more effectively. IFN-γ stimulation up-regulated the expression of immunosuppressive genes like IDO1, COX2, HLA-G, and soluble suppressive proteins such as HLA-G, KYN, IL10, PGE2 of MSC. And the immuno suppression capability of IFN-γ-stimulated MSC was 2-7 folds higher than control in MSC and lymphocyte co-culture tests. It is concluded that IFN-γ can effectively enhance the immunosuppressive capability of MSC.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Umbilical Cord
/
Cells, Cultured
/
Immunosuppression Therapy
/
Interferon-gamma
/
Cell Biology
/
Allergy and Immunology
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2014
Type:
Article