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The significance of different sample types in study of pandemic A (H1N1) influenza diagnosis / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 1079-1082, 2010.
Article in Chinese | WPRIM | ID: wpr-349919
ABSTRACT
<p><b>OBJECTIVE</b>To explore the value of different types of samples, including throat swabs, stools, bloods in pandemic A (H1N1) influenza diagnosis and virus shedding patterns.</p><p><b>METHODS</b>From May to June in 2009, 135 samples were collected from 23 confirmed cases of pandemic influenza A (H1N1) infection, including 99 throat swabs, 14 stools, 11 bloods, 1 respiratory tract washing from 13 confirmed cases and 10 blood samples from other confirmed cases. The virus was detected by real-time RT-PCR, the antibody was detected by haemagglutination inhibition assay.</p><p><b>RESULTS</b>For 99 throat swabs of 13 patients, the median time of the first positive real-time RT-PCR was 1 day (ranged from 0 to 7 days) after the onset of the symptoms of illness; the median length of time duration of positive real-time RT-PCR results from throat swabs was 3 days (ranged from 1 to 15 days). Four cases intermittently released virus. One respiratory tract washing sample was positive. In 14 stools, 8 stools were real-time RT-PCR positive, the positive rate was 57.14%. The median time of the positive real-time RT-PCR was 3 days (ranged from 1 to 4 days) after the onset of the symptoms of illness. In 21 blood samples collected at 2 to 9 days of onset, 1 blood sample was real-time RT-PCR positive, the positive rate was 4.76%. All these 21 blood samples were antibody negative.</p><p><b>CONCLUSION</b>Throat swabs and stools samples can be used as A (H1N1) influenza early diagnosis. The length of time duration of positive real-time RT-PCR in throat swabs was longer than stool samples and intermittently releasing of virus were found in throat swabs. Influenza A H1N1 cases showed the presence of small amount of viremia and antibody was negative in early blood samples (< 9 days).</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Hemagglutination Inhibition Tests / China / Epidemiology / Virus Shedding / Reverse Transcriptase Polymerase Chain Reaction / Diagnosis / Allergy and Immunology / Influenza, Human / Influenza A Virus, H1N1 Subtype Type of study: Diagnostic study / Screening study Limits: Adolescent / Adult / Child / Female / Humans / Male Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Preventive Medicine Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Hemagglutination Inhibition Tests / China / Epidemiology / Virus Shedding / Reverse Transcriptase Polymerase Chain Reaction / Diagnosis / Allergy and Immunology / Influenza, Human / Influenza A Virus, H1N1 Subtype Type of study: Diagnostic study / Screening study Limits: Adolescent / Adult / Child / Female / Humans / Male Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Preventive Medicine Year: 2010 Type: Article