Significant increase of glucose transport activity in breast cancer / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 103-108, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-349964
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression level and significance of glucose transporter 1 (Glut-1) in normal breast tissue, adenosis, adenoma and breast carcinoma.</p><p><b>METHODS</b>A total of 147 cases of female breast tissue samples, including 92 cases of invasive ductal carcinoma, 26 cases of breast fibroadenoma, 24 cases of breast adenosis and 5 cases of normal breast tissues, were collected for quantitative detection of the expression of Glut-1 protein by immunohistochemistry (EnVision method) and Western blot, and its mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>In normal breast tissue and benign lesions of the breast, Glut-1 was undetectable or only weakly detectable in cytoplasm of ductal and acinar epithelia. In contrast, the intensity of Glut-1 staining was significantly higher in invasive ductal carcinomas (P = 0.0002) with protein expression predominantly in cellular membrane and lesser in cytoplasm. Western blot and RT-PCR analyses showed that the expression of Glut-1 protein and mRNA were significantly increased in invasive ductal carcinoma than fibroadenoma (P =0.001 for protein; P <0.05 for mRNA) and adenosis (P =0.001 for protein; P < 0.05 for mRNA). There was a significant difference among groups (P = 0.0002 for protein; P = 0.0001 for mRNA).</p><p><b>CONCLUSIONS</b>Glucose transport activity, as indicated by Glut-1 protein and its mRNA expression, significantly increases in breast carcinoma than non-cancerous lesions. The over-expression of Glut-1 in breast carcinoma is tightly coupled with tumor cell proliferation, invasion and metastasis, implying that Glut-1 may serve as a new marker in the early diagnosis and prognostication of breast malignancy as well as a new therapeutic target.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Prognosis
/
Breast Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Carcinoma, Ductal, Breast
/
Glucose Transport Proteins, Facilitative
/
Glucose Transporter Type 1
/
Genetics
/
Glucose
/
Metabolism
Type of study:
Prognostic study
/
Screening study
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS