Clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue lymphoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 736-741, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-350026
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue (MALT) lymphoma.</p><p><b>METHODS</b>Six cases of MALT lymphoma with high-grade transformation were studied. Immunohistochemical study was carried out by EliVision using bcl-10 antibody. Reverse transcription-polymerase chain reaction was used to detect the presence of API2-MALT1 fusion gene transcripts. The target cells were selected by laser microdissection and studied by polymerase chain reaction and sequence analysis for rearrangement of immunoglobulin heavy chain gene.</p><p><b>RESULTS</b>In the 6 cases of MALT lymphoma with high-grade transformation, nuclear and cytoplasmic expression of bcl-10 was demonstrated in 1 case, while API2-MALT1 fusion gene was present in 2 cases. Identical fragments of rearranged immunoglobulin heavy chain gene were detected in both transformed and non-transformed components in each of the 6 cases, except for the differences at 2 nucleotide positions in N or D regions in 2 cases.</p><p><b>CONCLUSION</b>The tumor cells from both transformed and non-transformed components in MALT lymphoma derive from the same clone.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Base Sequence
/
Gene Rearrangement, B-Lymphocyte, Heavy Chain
/
Oncogene Proteins, Fusion
/
Cell Nucleus
/
Cell Transformation, Neoplastic
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
Lymphoma, B-Cell, Marginal Zone
/
Cytoplasm
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS