Preliminary studies on the mechanisms of a new anti-tumor agent PH II-7 with special preference to multidrug resistant tumor cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 134-139, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-350060
ABSTRACT
<p><b>OBJECTIVE</b>To determine the anti-tumor activity of PH II-7 in vitro and explore preliminarily its mechanisms.</p><p><b>METHODS</b>The anti-tumor activity was measured using colorimetric MTT assay. Apoptosis was determined with fluorescence-activated cell sorter (FACS), electron microscopy and agarose gel electrophoresis. The expressions of mdr1 and sorcin genes were determined by Northern blot assay.</p><p><b>RESULTS</b>PH II-7 inhibited the proliferation of various human tumor cells derived from different tumor cell lines. The IC50 values varied from 0.34-18.61 mumol/L. Especially, PH II-7 had strong inhibitory effect on multidrug resistant tumor cells, whereas adriamycin (ADR) was resistant. Apoptosis was induced in HL60 and HL60/ADR cells treated with 1 microgram/ml PH II-7, while PH II-7 inhibited the expressions of mdr1 and sorcin genes.</p><p><b>CONCLUSIONS</b>PH II-7 is a new potential agent which has strong inhibitory effect on both multidrug resistant cells and their parental cells. PH II-7 may increase the intracellular drug concentration in MDR cells by inhibiting the expressions of the MDR-related genes mdr1 and sorcin and induce the apoptosis of MDR cells and their parental tumor cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Cell Division
/
Chemistry
/
Apoptosis
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
HL-60 Cells
/
K562 Cells
/
Drug Combinations
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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