Model of B immunoblastic lymphomas in the Hu-PBL-SCID mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 294-296, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-350106
ABSTRACT
<p><b>OBJECTIVE</b>To constitute a model of B immunoblastic lymphomas in the Hu-PBL-SCID mice.</p><p><b>METHODS</b>The SCID mice were reconstituted by intraperitoneal injection (i.p.) of 5 x 10(7) human lymphocytes from Epstein-Barr virus (EBV) seronegative individuals. After one week, the SCID mice were inoculated with EBV by i.p. injection, and subjected to the investigation of whether there was any tumor in the abdomen of such SCID mice four weeks later. The characteristics of the found tumor was observed by the methods of Hematoxylin-eosin (HE) stain, immunohistochemical staining and polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Compared with the control groups, all the EBV-infected Hu-PBL-SCID mice had abdominal solid tumors [(32 +/- 12.5) mm3] developed, often located in the liver. HE staining and immunohistochemical staining showed the tumors were human B cell lymphomas. EBV DNA could be detected in the tumors by the PCR.</p><p><b>CONCLUSIONS</b>The model of B immunoblastic lymphomas in the Hu-PBL-SCID mice is successfully constituted, and may well be useful to the human tumor immunological study.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Lymphoma, Large-Cell, Immunoblastic
/
Mice, SCID
/
Herpesvirus 4, Human
/
Disease Models, Animal
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article
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