Accurate identification of Psammosilene tunicoides and its confused species by systematic identification method / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1134-1137, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350645
ABSTRACT
<p><b>OBJECTIVE</b>To develop an effective identification method for accurately discriminating Psammosilene tunicoides and its confused species by the combined method of microscopic identification and molecular identification, so-called systematic identification of Chinese materia medica (SICMM).</p><p><b>METHOD</b>P. tunicoides and its confused species were accurately discriminated by SICMM method, which was established by comprehensively use of microscopic identification and DNA identification method. The DNA identification included the following analysis:
the BLAST alignment, specific bases and N-J phylogenetic tree analysis.</p><p><b>RESULT</b>The cluster crystals were not observed in P. tunicoides, but great deals of them were found in Silene viscidula. Further more, big differences of ITS sequence were observed and analyzed between P. tunicoides and its confused specie of S. viscidula.</p><p><b>CONCLUSION</b>The system method is a scientific and accurate method for the identification of P. tunicoides and its counterfeit species.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Phylogeny
/
Base Sequence
/
Chemistry
/
Sequence Alignment
/
Classification
/
DNA, Intergenic
/
Caryophyllaceae
/
Cell Biology
/
Genetics
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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